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KMID : 0378019700130040063
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New Medical Journal
1970 Volume.13 No. 4 p.63 ~ p.68
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Population Variance of Entamoeba histolytica and Trichomonas hominis in a modified Diphasic Medium
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鄭坪林/Chung, Pyung-Rim
張在景/曺泳善/李定熙/Chang, Jae-Kyong/Cho, Yong-Seun/Lee, Chung-Hee
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Abstract
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This study was designed to investigate whether two population groups of. E. lzistolytica and T. homi,Fis exist together in the same ecological environment or not.
Two protozoan parasites were cultivated at once in modified diphasic medium (Faust & Russell, 1964), and checked the trends of population variance daily or two-days interval. Sodium and potassium contents, and pH levels in the media were respectively measured.
The results are summarized as follows:
1. In the media in which Ea hislolytica and Ts honzinis were cultured simultaneously, the former annihilated on the third subculture and yet the latter showed continuous increase.
2. In the media in which the single species was cultured, both E. histolytica and T. honzinis multiplied increasingly, though the latter was more conspicuous.
3. In the mixed group without subculture, all the Ea histolytica diminished within 96 hours, while Ts hominis survived even until 144 hours. The performation of subculture for Ts hominis may be permitted in modified diphasic medium once a week.
4. In the single culture groups without subculture, E. histolytica disappeared within 144 hours, but Ts hominis survived up to 144 hours.
5. In the media containing Ts hominis the amounts of sodium and potassium increased to higher level at 144 hours to compare the value at 24 hours, whereas the amounts in the media containing Ea histolytica decreased gradually.
As shown in above results, the mixei cultivation with E.histolylica and Ts hominis was unadvisable in modified diphasic medium due to more exceptional multiplication of T. hominis than E. histolytica.
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